重组人碱性成纤维细胞生长因子（bFGF）技术转让

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英 文 名：
Recombinant Human Basic Fibrobast Growth Factor
英文缩写：
FGF、rhFGF、rhuFGF、rh-FGF、rhu-FGF、 basic Fibrobast Growth Factor
中 文 名：
重组人碱性成纤维细胞生长因子，重组人碱性成纤维生长因子，人碱性成纤维生长因子
来    源：
毕赤酵母（真核细胞）
描述：
重组人碱性成纤维细胞生长因子（bFGF）由146个氨基酸组成的相对分子量为16kD的多肽。采用基因工程技术和独特的色谱技术分离纯化、冻干制备。bFGF具有强有力的促进细胞分裂增殖的生物活性。
品质:
外观：白色疏松状冻干粉。
纯度：>95% ，采用反相-高效液相（RP-HPLC）方法测定。
纯度：>95% ，采用还原型聚丙烯酰胺凝胶电泳（SDS-PAGE），银染检测。
生物活性：2×105 IU/mg.
PH值：7.2
包装:
0.1mg、1mg、2 mg、10mg/西林瓶，粉状冻干品密封包装。
储存:
bFGF冻干粉在室温下可存放180天；建议在2-8℃的冷柜中避光、密封保存，三年其生物活性不改变。
用途：
bFGF通过与细胞膜上的受体，广泛作用于外胚层、内胚层和中胚层多种来源的细胞，包括上皮细胞、粘膜细胞、内皮细胞、平滑肌细胞、骨骼肌细胞、卵巢颗粒细胞、成纤维细胞和神经细胞等，都具有促丝裂原作用和趋化作用，是一种与组织创伤的愈合过程密切相关的内源性活性因子。主要作用机制为：
l）趋化作用：rh-bFGF作为化学趋化剂趋化多种炎性细胞、组织细胞与修复因子，向创面或伤口迁移，为创面杀菌以及后期修复创造重要和必需的条件。
2）增殖作用：①rh-bFGF直接作用于组织修复细胞上的生长因子受体，通过其促分裂效应加速细胞周期转变来加速创面修复；②rh-bFGF通过竞争性作用上调组织修复细胞上生长因子受体的活性，从而加快信号的传递。
3）重建作用：bFGF通过增加细胞内胶原蛋白、连接蛋白、透明质酸、羟脯氨酸、糖蛋白的合成，提高各种再生细胞间的连接强度和修复质量，减少和预防疤痕的形成。
应用领域：
作为生物功效性原料添加以下产品中使用。
① 抗皱产品：激活细胞代谢，促胶原纤维合成，达到抗皱效果；
② 修复产品：促进皮下神经和血循环组织的再建，增强修复效果。
医药领域。重组人成纤维细胞生长因子（rh-bFGF）作为国际上最先进的第二代细胞生长因子，在下列
临床市场领域有广泛应用：
●烧烫伤            ●妇产科             ●难愈性创面
●口腔溃疡          ●创伤外科           ●手术切口愈合
●放疗科            ●激光、美容医学     ●换药室
生化试剂
本品可为生化试剂在科研、检测等多个领域中使用。


Recombinant Human Basic Fibrobast Growth Factor

Product Name:
Recombinant Human Basic Fibrobast Growth Factor
Synonyms:
FGF、rhFGF、rhuFGF、rh-FGF、rhu-FGF、 basic Fibrobast Growth Factor
Description:
Basic fibroblast growth factor possess broad mitogenic and cell survival activities,and are involved in a variety of biological processes, including embryonic development、cell growth、morphogenesis、tissue repair、tumor growth and invasion. Recombinant Human Basic Fibrobast Growth Factor produced in P.pastoris, polypeptide chain containing 155 amino acids and having a molecular mass of 17353 Dalton.The Bfgf is purified by gene techniques and proprietary chromatographic techniques.
Source:
P.pastoris
Physical Appearance:
White lyophilized（freeze-dried）powder
Purity:
Greater than 95% as determined by:
(a)        Analysis by RP-HPLC.
(b)        Analysis by SDS-PAGE.
Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Glu-Gly-Glu-Ile, which agrees with the sequence of native human bFGF.
Biological Activity:
The ED50 calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors is less then 0.5ng/ml corresponding to a specific activity of 2×105 Units/mg.
Packing:
In Penicillin vial, inner sealed with 0.1mg,、1mg、2mg、10mgfreeze-dried power.
Storage:
Lyophilized Epidermal Growth Factor although stable at room temperature for 18 days; and stored at 2-8°C for 3 years.
Usage:
Through the cell membrane receptors , bFGF extensively acts on ectoderm, endoderm and mesoderm cells of a variety of sources, including epithelial cells, mucosal cells, endothelial cells, smooth muscle cells, skeletal muscle cells, ovarian granulosa cells, fibroblasts and nerve cells and so on, have a role in promoting mitogen and chemoattractant, is a endogenous factors which is closely related to the tissue healing process.The main mechanism is as following:
l) Chemotaxis:As a chemoattractant, rh-bFGF drives multiple inflammatory cells, tissue cells and repair factor towards  the wounds or the injury ,creating important and necessary conditions for wound sterilization and late repair.
2) proliferation:①rh-bFGF directly acts on the growth factor receptor located on tissue repair cells, through its mitogenic effect to accelerate cell cycle changes to accelerate wound healing; ②rh-bFGF through competitive effect increase the activity of growth factor receptor located on tissue repair cells, sequentially accelerating the signal transduction.
3) reconstruction: bFGF by increasing the synthesis of intracellular collagen,fibronectin,hyaluronic acid,hydroxyproline and glycoprotein enhances the connection intensity and repair quality among various renewable cells result in reducing and preventing scar formation.
Application areas:
as a biological efficacy material addedto the following products:
① Anti-Wrinkle Products: activation of cell metabolism, promoting synthesis of collagen fibers to anti-wrinkle effects;
②repair Products: promote the reconstitution of subcutaneous nerves and circulation organizations and enhance repair effects.
the field of medicine. As  the most advanced second generation cell growth fator internationally, rh-bFGF is widely used in the following clinical areas:
●burn and scald wound           ●Obstetrics and Gynecology            
●refractory wound         ●Oral ulcer              
●Trauma Surgery          ●surgical wound healing
●Radiotherapy           ●lasers, cosmetic medical     ●Dressing Room
Biochemical reagent:
This product can be used as biochemical reagent in research, detection and many other various fields.