表皮细胞生长因子技术转让

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英文名：
Recombinant Human Epidermal Growth Factor
英文缩写：
EGF、rhEGF、rhuEGF、rh-EGF、rhu-EGF、Epidermal Growth Factor
中文名：
重组人表皮细胞生长因子，重组人表皮生长因子，表皮生长因子，人表皮生长因子
描 述：
重组人表皮生长因子（EGF）是由53个氨基酸组成的相对分子量为6222的小分子多肽。采用基因工程技术和独特的色谱技术分离纯化、冻干制备。EGF作为一种强有力的细胞分裂因子，具有多种生物活性。
来源：
毕赤酵母
品质:
外观：白色疏松状冻干粉。
纯度：>95% ，采用反相-高效液相（RP-HPLC）方法测定。
纯度：>95% ，采用非还原性聚丙烯酰胺凝胶电泳（SDS-PAGE），银染检测。
生物活性：5×105 IU/mg.
PH值：7.2
包装:
0.1mg、1mg、2 mg、10mg、1g/西林瓶，粉状冻干品密封包装。
储存:
EGF冻干粉在室温下可存放180天；建议在2-8℃的冷柜中避光、密封保存，三年其生物活性不改变。
用途:
重组人表皮生长因子（EGF）作为一种强有力的细胞分裂因子，具有多种生物活性，EGF具有修复表皮、抗衰老、淡化色斑、平复皱纹、滋润之功效，人体中EGF的含量多少直接决定着皮肤的年轻程度，EGF因此又被誉为“美丽因子”，主要表现为：
1）在体内刺激皮肤组织、角膜和气管上皮组织的生长繁殖。
2）促进细胞再生和组织修复：EGF有促进皮肤和黏膜创伤面的愈合作用，并减少疤痕挛缩和皮肤畸形增生，加速角膜、皮肤等表皮创伤的修复。用于治疗疤痕，尤其是痤疮后留下的疤痕、疙瘩疗效较好。
3）嫩肤护肤作用：EGF能促进皮肤细胞对营养物质的吸收，加速细胞的新陈代谢，促进皮肤细胞的分裂和增长，促进透明质酸和糖蛋白的合成。
4）增强表皮细胞的蛋白质、DNA、RNA的合成和细胞代谢等。
5）防晒作用：EGF能促进新生细胞生长，替代受紫外线照射损伤的细胞，以降低皮肤中黑色素细胞的数量。
6）EGF不仅具有促进皮肤和黏膜创伤的愈合，防治溃疡以及消炎镇痛的作用，而且能有效促进和调节表皮细胞的生长和增殖，表皮细胞生长因子对保护和疗养皮肤、黏膜具有十分独特的功效。广泛应用于治疗烧伤、烫伤、手术伤、机械伤、皮肤溃疡、激光美容等。
应用范围:
医药：表皮生长因子在医药领域具有重要的意义，研究表明它能促进受伤的皮肤组织生长，抑制胃酸的分泌，可促进烧伤皮肤的生长和皮肤溃疡的愈合，它将在皮肤病、胃病治疗、角膜移植手术中起重要作用。
化妆品：美国学者的研究表明，表皮生长因子具有延缓皮肤细胞衰老、促使人皮细胞的修复和生长的作用，使皮肤光滑丰润。
生化试剂: 本品可为生化试剂在科研、检测等多个领域中使用。

Recombinant Human Epidermal Growth Factor

Product Name:
Recombinant Human Epidermal Growth Factor
Synonyms:
EGF、rhEGF、rhuEGF、rh-EGF、rhu-EGF、 Urogastrone、URG、
Description:
EGF is a small polypeptide containing 53 amino acids and having a molecular mass of 6222 Dalton. It is gene engineering product .It is purified by proprietary chromatographic techniques and prapared by Freeze-dry techniques.
Source:
P.pastoris
Physical Appearance:
White lyophilized（freeze-dried）powder
Purity:
Greater than 95% as determined by:
(a)        Analysis by RP-HPLC.
(b)        Analysis by SDS-PAGE.
Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu, which agrees with the sequence of native human EGF.
Biological Activity:
The ED50 calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 0.1ng/ml corresponding to a specific activity of 1×107 Units/mg.
Packing:
In Penicillin vial, inner sealed with 0.1mg、1mg、2mg、10mg、1g freeze-dried power.
Storage:
Lyophilized Epidermal Growth Factor although stable at room temperature for 18 days; and stored at 2-8°C for 3 years.
Usage:
As a highly efficient cell division factor ,EGF has many biological effects, e.g repair of Epidermal cell, anti-aging protection trating face splash elimination of wrinkles and moisturing .The content of EGF in body determines the young degree of skin directly. So, EGF is called “beauty fator”,which mainly includes:
-        Stimulating the growth and proliferation of skin, cornea and tracheal epithelium tissue in vivo.
-   Promoting cell regeneration and tissue repair. EGF can promote the wounds healing of skin and mucosa as well as decreasing scar contracture and skin malformation hyperplasia and accelarating the epidermal wounds repairation of cornea and skin. It is well used to treat scar, especially those left by acne.
-        Skin rejuvenation and protection: EGF can promote the skin cells to absorb nutrients and accelerate cell metabolism. At the same time, EGF can boost division and growth of skin cell as well as the synthesis of hyaluronic acid and glycoprotein.
-        Enhance the biosynthesis of protein, RNA, DNA as well as metabolic activity in epidermal cells.
-        Sunscreen role: EGF can promote the growth of neogenesis cell and replace the cell damaged by UV,which can reduce the number of melanocytes.
-        Widely used in the treatment of burns, surgical trauma, mechanical injury, skin ulcer, laser beauty. EGF not only has the promotion of skin and mucosal healing, ulcer prevention, as well as the role of anti-inflammatory analgesic,but also has the effective promotion and regulation of epidermal cell growth and proliferation . EGF has a unique effect on protection and convalescence of skin.
Application scoppe:
Medicine: EGF is of great significance in the field of medcine. The study shows that it can promote the growth of injured skin tissue, inhibiting gastric acid secretion, it can also promote the growth and heal of burned and ulcered skin. It will play an important role in dermatosis, Gastropathy treatment and corneal transplant surgery.
Cosmetics: United States scholars have shown that EGF can delay the skin cell aging and promote human skin cell repair and growth allowing the skin smooth and rich.
Biochemical reagent:
This product can be used as biochemical reagent in research, detection and many other various fields.